Purification of FXACplicMBP from 2L of Terrific broth medium 1Oct

Elution 1 and 2 - 5 ml and 5 ml mixed and injected in S75 column. 

Peak 1 fractions 4,5,6,7,8 (2ml each) concentrated to around 6.5 mg/ml and set up Crystal Screen 1. 

             UL          lysate     pellet      flowthr   BBW   Wash1   BAW     Eln1         Eln2        BAE