Saturday, October 1, 2016

FXAC - SUMO and FXAC NUS expression check


                                         UI                I            UI            I              UI           I              I        UI


Pageruler prestained      FXAC-SUMO             SUMO                 FXAC-NUS               NUS

Culture volume - 4 ml of BL21 cells induction for 3 hours at 37 degrees with 2ul of 1M IPTG in 3ml of culture.

Cloning - both in Eco Xho sites


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