Factor Xa directed Anticlotting serpin like protein (Antithrombin)
Saturday, October 1, 2016
FXAC - SUMO and FXAC NUS expression check
UI I UI I UI I
I
UI
Pageruler prestained
FXAC-SUMO SUMO FXAC-NUS NUS
Culture volume - 4 ml of BL21 cells induction for 3 hours at 37 degrees with 2ul of 1M IPTG in 3ml of culture.
Cloning - both in Eco Xho sites
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