Factor Xa directed Anticlotting serpin like protein (Antithrombin)
Tuesday, October 11, 2016
FXAC plic MBP and FXAC pet 28 SUMO culture
Inoculated 2l of FXAC plic MBP in LB amp and 2l Terrific Broth FXAC pet28 SUMO in LB Kan 4 PM.
Added 500ul of 1M IPTG at 7:30 PM at OD of 1.2 (TB) and 0.8(LB)
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