Factor Xa directed Anticlotting serpin like protein (Antithrombin)
Wednesday, October 12, 2016
FXAC plicMBP purification 13Oct 2L
Sonication 50 min. Spin down at 2pm at 18k RPM.
Amylose resin 5ml equilibrated.
2:40 pm - 1h binding in cold room.
prestained plus ladder, BAW, Elution1, Elution2, BAE
Set up additive screen with this (CS1-12) at 9mg/ml and 4.5 mg/ml
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