Wednesday, October 12, 2016

FXAC plicMBP purification 13Oct 2L

Sonication 50 min. Spin down at 2pm at 18k RPM.
Amylose resin 5ml equilibrated.
2:40 pm - 1h binding in cold room.

prestained plus ladder, BAW, Elution1, Elution2, BAE

Set up additive screen with this (CS1-12) at 9mg/ml and 4.5 mg/ml
                  

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