Monday, October 31, 2016

fxac mbp 2l and fxac sumo 1l Terrific broth purification 31 oct

FXAC MBP and FXAC pet28 SUMO (BAW, E1, E2, BAE)



FXAC MBP run 2: Run 1 had a nice peak of 250 mAU but lost sample as tubes were not kept in the fraction collector. So I collected the spillage and concentrated to 10ml and injected again to get this small peak. Precipitation was also observed after spillage

FXAC MBP run 3 Third elution was done with 400mM Maltose in 5ml total volume. There was not much protein left in the beads. 
fxacpet28sumo fplc (below curve) fractions A4 to A11

FXAC pet28 SUMO : 10ml of elution from yesterday was injected in the early morning. There was a huge peak of 300 for imidazole in the 120ml range.







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