Measured today but due to salt and glycerol, readings showed 9kDa peptides.
50ul of protein was passed through 10kDa cutoff centricon several times with HPLC buffer A.
Tomorrow will measure the ESI MS mass again.
13 Oct
Measured the mass spec mass again. Major peak was a 16kDa protein. This was not conclusive. Will repeat with the department mass spec facility or repeat western.
50ul of protein was passed through 10kDa cutoff centricon several times with HPLC buffer A.
Tomorrow will measure the ESI MS mass again.
13 Oct
Measured the mass spec mass again. Major peak was a 16kDa protein. This was not conclusive. Will repeat with the department mass spec facility or repeat western.
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