Sonicated pellet with 50ml lysis buffer and pmsf for 50 min.
Binding to 5ml Amylose resin (3pm to 4pm)
Concentrated FPLC fractions to 70ul and set up PEGRx1 and 2 screens
Also set up optimization for Index condition 44 for spherulite, CS2 31 and CS1 condition 12.
Binding to 5ml Amylose resin (3pm to 4pm)
Concentrated FPLC fractions to 70ul and set up PEGRx1 and 2 screens
Also set up optimization for Index condition 44 for spherulite, CS2 31 and CS1 condition 12.
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| FPLC of elution 1 10 ml , 500 mM imidazole |
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| FPLC of elution 2 , 5ml , 500mM Imidazole |
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| SDS gel of Run1 fractions |
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