Primer list

1)  antig5-for-pLICMBP  

5’ CCAGGGAGCAGCCTCG GAATTCG CTGGAAGTGCT  3’

wrong primer. out of frame restriction site

2) antig5-rev-pLICMBP

5’ GCAAAGCACCGGCCTCGTTA CTCGAGTTACGGGTTGTTGCTCAC 3’

3) antig5-Rev-pet2O-T  

5'  TTATCCACTTCCAATGTTATTA CTCGAGTTACGGGTTGTTGCTCAC 3'

wrong primer : do not include Restriction site CTCGAG after fusion tag

4) angio-rev-pet2OT

5'  TTATCCACTTCCAATGTTATTA CTCGAGTTACTCCGCGGTCTTCAG 3'

5)FXAC-For-pet2O-T  

  5'TACTTCCAATCCAATGCA ATGGCGATCGACTACCAGGC 3' 

6)FXAC-Rev-pet2O-T 

  5'TTATCCACTTCCAATGTTATTA TTAGGTCACCATGTGGCCAACAATG 3

7) 23.5CF-For-pet2O-T 

5'TACTTCCAATCCAATGCA GAATTCGCTGGAAGTTCT 3'

wrong primer :  do not include restriction site GAATTCG after fusion tag

8) 23.5CF-Rev-pet2O-T 

5'TTATCCACTTCCAATGTTATTA CTCGAGTTAGCAATACAGTTCGGTA 3' 

9) 23.5CF-for-pLICMBP  

5’ CCAGGGAGCAGCCTCG GAATTCGCTGGAAGTTCT  3’

wrong primer :  do not include restriction site GAATTCG after fusion tag

10) 23.5CF-rev-pLICMBP

5’ GCAAAGCACCGGCCTCGTTA CTCGAGTTAGCAATACAGTTCGGTA  3’

11) For Fxac pmalc5x NdeI

GATCGATC CAT ATG ATG GCG ATC GAC TAC CA