ProtParam
User-provided sequence:
10 20 30 40 50 60
MKSIISITIT VLAIICEGQA TNYCDPSLCA RGTPHIACNG LSTLSRTCGA GSFEVALNRA
70 80 90 100 110 120
DQQLIVDLHN KLRSKVAMGQ QKNSAGQRFQ QACRMATLQW DPELAHIAAT NARRCVYGHD
130 140 150 160 170 180
RCRNTASMKF AGQNIAIKYY YGMTFTNEQL LTGFINSWFS EFKDATPQQI GNVCSGCITG
190 200 210 220 230 240
CNRVFPGLCN TAERVSNNPA RYPANYRGPA IGHFTQIVSD RTSRIGCSMV SYNKNGFINK
250
LFVCNYGVTN IINQPVYVA
Number of amino acids: 259
Molecular weight: 28479.55
Theoretical pI: 9.18
Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1238
Hydrogen H 1953
Nitrogen N 365
Oxygen O 368
Sulfur S 20
Formula: C1238H1953N365O368S20
Total number of atoms: 3944
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 26775
Abs 0.1% (=1 g/l) 0.940, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 0.909, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.75
This classifies the protein as stable.
Aliphatic index: 76.14
Grand average of hydropathicity (GRAVY): -0.151
Number of amino acids: 259 Molecular weight: 28479.55 Theoretical pI: 9.18
PROTEIN PARAMETERS OF ANTIGEN5 MBP FUSION PROTEIN
Total number of negatively charged residues (Asp + Glu): 64 Total number of positively charged residues (Arg + Lys): 71 Atomic composition: Carbon C 3210 Hydrogen H 5025 Nitrogen N 865 Oxygen O 949 Sulfur S 29 Formula: C3210H5025N865O949S29 Total number of atoms: 10078 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 93125 Abs 0.1% (=1 g/l) 1.296, assuming all pairs of Cys residues form cystines Ext. coefficient 92250 Abs 0.1% (=1 g/l) 1.284, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 23.20 This classifies the protein as stable. Aliphatic index: 81.73 Grand average of hydropathicity (GRAVY): -0.212MKSIISITIT VLAIICEGQA TNYCDPSLCA RGTPHIACNG LSTLSRTCGA GSFEVALNRA 70 80 90 100 110 120 DQQLIVDLHN KLRSKVAMGQ QKNSAGQRFQ QACRMATLQW DPELAHIAAT NARRCVYGHD 130 140 150 160 170 180 RCRNTASMKF AGQNIAIKYY YGMTFTNEQL LTGFINSWFS EFKDATPQQI GNVCSGCITG 190 200 210 220 230 240 CNRVFPGLCN TAERVSNNPA RYPANYRGPA IGHFTQIVSD RTSRIGCSMV SYNKNGFINK 250 260 270 280 290 300 LFVCNYGVTN IINQPVYVAM KIKTGARILA LSALTTMMFS ASALAKIEEG KLVIWINGDK 310 320 330 340 350 360 GYNGLAEVGK KFEKDTGIKV TVEHPDKLEE KFPQVAATGD GPDIIFWAHD RFGGYAQSGL 370 380 390 400 410 420 LAEITPDKAF QDKLYPFTWD AVRYNGKLIA YPIAVEALSL IYNKDLLPNP PKTWEEIPAL 430 440 450 460 470 480 DKELKAKGKS ALMFNLQEPY FTWPLIAADG GYAFKYENGK YDIKDVGVDN AGAKAGLTFL 490 500 510 520 530 540 VDLIKNKHMN ADTDYSIAEA AFNKGETAMT INGPWAWSNI DTSKVNYGVT VLPTFKGQPS 550 560 570 580 590 600 KPFVGVLSAG INAASPNKEL AKEFLENYLL TDEGLEAVNK DKPLGAVALK SYEEELAKDP 610 620 630 640 650 RIAATMENAQ KGEIMPNIPQ MSAFWYAVRT AVINAASGRQ TVDEALKDAQ TRITK
Number of amino acids: 655 Molecular weight: 71849.18 Theoretical pI: 8.54
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