Tuesday, September 13, 2016

FactorXa directed anticlotting serpin like protein - constructs available

FXAC pGEX6P1 Eco Xho
FXAC pet28SUMO Eco Xho
FXAC pet32a with thioredoxin tag - expression not good - Restriction sites - EcoRI and XhoI and precission site before start codon
FXAC plic MBP   - Expression good - TEV cleavage is not suitable
FXAC pet2OT with MOCR tag - Still in DH5 alpha
FXAC pet 44 with NUS tag
FXAC pet 28 - no expression - NcoI and XhoI



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