ProtParam
User-provided sequence:
10 20 30 40 50 60
MYLKVVILFS LQLVCYTQGG TPPSKPMAID YQAEFAWDLY KKLQLGFTQN LAIAPYSLRK
70 80 90 100 110 120
IFVCLQQLTD SENPASAALS EQLKNVFKFN PKGKLPDLVR SRYSAQWTAL EREKGINTTT
130 140 150 160 170 180
LAAVIGREKK TKLFRDLPNS CAIFAASLTP GSPKQMSRWF NAAMRNISKS GVQNFLSTSD
190 200 210 220 230 240
IDRDWDFLVA DSWIFKGLWR YQFEEEHKTT CNFYTNSTSK GLMRFMYLQE YLKHGYFLEW
250 260 270 280 290 300
NVQAVELPLH HGSSFSCMLM MPVKADIGVL IKSLNHRRFK DIYSKMSVSK TDVRLPQFTL
310 320 330 340 350 360
RIKLSAKSIL QQFGFNAAFN ESVFHVFDNK NAVPLGDVIQ KVKLVMDHDG EQSAKMYVDR
370 380 390 400 410
RMGNLFIAHQ QFIFVIFEKT QLVPIIVGHM VTASTPKDIG PESDEISCDK PPRY
References and documentation are available.
Total number of negatively charged residues (Asp + Glu): 38 Total number of positively charged residues (Arg + Lys): 51 Atomic composition: Carbon C 2155 Hydrogen H 3346 Nitrogen N 566 Oxygen O 598 Sulfur S 20 Formula: C2155H3346N566O598S20 Total number of atoms: 6685 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 59735 Abs 0.1% (=1 g/l) 1.260, assuming all pairs of Cys residues form cystines Ext. coefficient 59360 Abs 0.1% (=1 g/l) 1.253, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 40.09 This classifies the protein as unstable. Aliphatic index: 84.08 Grand average of hydropathicity (GRAVY): -0.196
Number of amino acids: 414 Molecular weight: 47392.91 Theoretical pI: 9.31
Protparam for MBP FXAC fusion
MKIKTGARIL ALSALTTMMF SASALAKIEE GKLVIWINGD KGYNGLAEVG KKFEKDTGIK
70 80 90 100 110 120
VTVEHPDKLE EKFPQVAATG DGPDIIFWAH DRFGGYAQSG LLAEITPDKA FQDKLYPFTW
130 140 150 160 170 180
DAVRYNGKLI AYPIAVEALS LIYNKDLLPN PPKTWEEIPA LDKELKAKGK SALMFNLQEP
190 200 210 220 230 240
YFTWPLIAAD GGYAFKYENG KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE
250 260 270 280 290 300
AAFNKGETAM TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE
310 320 330 340 350 360
LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA QKGEIMPNIP
370 380 390 400 410 420
QMSAFWYAVR TAVINAASGR QTVDEALKDA QTRITKMYLK VVILFSLQLV CYTQGGTPPS
430 440 450 460 470 480
KPMAIDYQAE FAWDLYKKLQ LGFTQNLAIA PYSLRKIFVC LQQLTDSENP ASAALSEQLK
490 500 510 520 530 540
NVFKFNPKGK LPDLVRSRYS AQWTALEREK GINTTTLAAV IGREKKTKLF RDLPNSCAIF
550 560 570 580 590 600
AASLTPGSPK QMSRWFNAAM RNISKSGVQN FLSTSDIDRD WDFLVADSWI FKGLWRYQFE
610 620 630 640 650 660
EEHKTTCNFY TNSTSKGLMR FMYLQEYLKH GYFLEWNVQA VELPLHHGSS FSCMLMMPVK
670 680 690 700 710 720
ADIGVLIKSL NHRRFKDIYS KMSVSKTDVR LPQFTLRIKL SAKSILQQFG FNAAFNESVF
730 740 750 760 770 780
HVFDNKNAVP LGDVIQKVKL VMDHDGEQSA KMYVDRRMGN LFIAHQQFIF VIFEKTQLVP
790 800 810
IIVGHMVTAS TPKDIGPESD EISCDKPPRY
References and documentation are available.
Total number of negatively charged residues (Asp + Glu): 89 Total number of positively charged residues (Arg + Lys): 97 Atomic composition: Carbon C 4127 Hydrogen H 6418 Nitrogen N 1066 Oxygen O 1179 Sulfur S 29 Formula: C4127H6418N1066O1179S29 Total number of atoms: 12819 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 126085 Abs 0.1% (=1 g/l) 1.389, assuming all pairs of Cys residues form cystines Ext. coefficient 125710 Abs 0.1% (=1 g/l) 1.385, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 29.82 This classifies the protein as stable. Aliphatic index: 84.72 Grand average of hydropathicity (GRAVY): -0.223
Number of amino acids: 810 Molecular weight: 90762.53 Theoretical pI: 8.72
No comments:
Post a Comment