protein parameters for angiopoietin

ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNRQLWIIIF AILCVAQAEE DNPTTEKMEE LGIATINNFT REFYSYVEAV SQVLADLELT 

        70         80         90        100        110        120 
TTASITQIKH RIKHLLQEKC NLCSAKAEGP ALDQGYVTTS NGSVIPVSYE QTRFGGGWIV 

       130        140        150        160        170        180 
LMQRYDGTVR FNRSWAEYRD GFGMVGHEFW LGLERIHQMT KDAEYELMIE MQDFEGNYKY 

       190        200        210        220        230        240 
AGYDAFAVGP EEERYPLAKV GKFNKTAYVD SFGKHRGYGF STYDNDDNGC SNQYGRGGWW 

       250        260        270        280        290 
YYRKSCFGAS LTGIWQNKQD WKSISWVWFS TEKKQVPLKF ARMMMRLKTA E 

Number of amino acids: 291

Molecular weight: 33633.01

Theoretical pI: 6.03


Amino acid composition: 
Ala (A)  20   6.9%
Arg (R)  15   5.2%
Asn (N)  13   4.5%
Asp (D)  13   4.5%
Cys (C)   5   1.7%
Gln (Q)  14   4.8%
Glu (E)  23   7.9%
Gly (G)  25   8.6%
His (H)   5   1.7%
Ile (I)  15   5.2%
Leu (L)  18   6.2%
Lys (K)  18   6.2%
Met (M)  10   3.4%
Phe (F)  15   5.2%
Pro (P)   6   2.1%
Ser (S)  16   5.5%
Thr (T)  18   6.2%
Trp (W)  10   3.4%
Tyr (Y)  17   5.8%
Val (V)  15   5.2%
Pyl (O)   0   0.0%
Sec (U)   0   0.0%

 (B)   0   0.0%
 (Z)   0   0.0%
 (X)   0   0.0%

Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C       1513
Hydrogen    H       2273
Nitrogen    N        401
Oxygen      O        442
Sulfur      S         15

Formula: C1513H2273N401O442S15
Total number of atoms: 4644

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    80580
Abs 0.1% (=1 g/l)   2.396, assuming all pairs of Cys residues form cystines


Ext. coefficient    80330
Abs 0.1% (=1 g/l)   2.388, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.21
This classifies the protein as stable.



Aliphatic index: 66.05

Grand average of hydropathicity (GRAVY): -0.488