Monday, December 19, 2016

fxacmbp and 23mbp purification using his tag affinity

FXAC and 23 MBP lysate, BAW, Eln1(2ml) and Eln2 (2ml)


FPLC fractions A6 to A13 on S200 (2ml injection) of elution 1 (FXACMBP)

SDS gel of above fplc run 



FXAC elution 2, 3, 4 (each 2ml ) was mixed and run on S200(19Dec). Gel was run of fractions A3, A4 , A5, A6, A7, A8, A9, A10, A11 (20 dec)


sds gel of above fplc peaks


23MBP elutions 2, 3, 4 were mixed and run on S200(20 Dec). Gel of fractions A6 to A13 was run. (20 Dec)

sds gel of the above fplc peak

23mbp run2 s200
no gel run

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