Dengue research team

peg ion and sg1 hits

0.2

M

Lithium chloride

20

% w/v

Polyethylene glycol 3,350

0.2

M

Calcium acetate hydrate

20

% w/v

Polyethylene glycol 3,350

7.5

0.2 M Magnesium chloride hexahydrate 0.1 M Sodium HEPES 7.5 25 % w/v PEG 3350

Salt crystals from wizard 1 34 optimization

wizard 1 (25-48) screening

Wizard 1 seeding with 3 different seeds (30 nov, cs12 and 27dec additive )

fxac seeding wizard2 pegion1 and sg1

0.2 M Lithium sulfate 0.1 M Tris 8.5 30 % w/v PEG 4000

0.2 M Calcium chloride dihydrate 20 % w/v PEG 3350

0.2 M Calcium chloride dihydrate 0.1 M Sodium HEPES 7.5 28 % v/v PEG 400

1.5 M Lithium sulfate 0.1 M Sodium HEPES 7.5

20% (w/v) PEG 1000 100 mM Sodium cacodylate/ Hydrochloric acid pH 6.5 200 mM Magnesium chloride