23.5 kDa culicine family protein expression check

Right to left first 3 lanes are colony 2 of 23cfplicmbp, next 3 are colony 12 which was negative and last 3 are for plicmbp control. 

western blot of the 23cf colony 2 lys, baw, elution 1:1000 anti MBP and 1:10000 anti mouse antibodies

1)  From 100ml and purification with amylose beads

2) Purified with 1XPBST buffer

3) gel lanes are lysate, beads after wash and elution

4) elution with 100mM Maltose + 1XPBST