MIDAS screen for FXAC MBP , 23 MBP and Antigen 5 MBP (ALL CRYSTALS ARE SALT)

0.1MPotassium/sodium phosphate 7.0 35% v/v Polypropylene glycol 400  SALT

0.1MLithium sulfate 0.1MTris8.0 25% v/v Jeffamine® ED-2003

0.1MPotassium/sodium phosphate7.0 14% v/vJeffamine® ED-900 11% v/v Jeffamine® SD-2001

0.2MSodium malonate dibasic monohydrate 0.1MMES 5.5 20% v/vJeffamine® D-2000

0.2MSodium malonate dibasic monohydrate 0.1MMES 5.5 20% v/vJeffamine® D-2000

0.2MCalcium chloride dihydrate 0.1MHEPES6.5 35% v/v Pentaerythritol ethoxylate (15/4 EO/OH)

THESE WERE USED AS SEED STOCK FOR SG1 SCREEN 

0.2MSodium sulfate 0.1M Tris8.0 20% w/v SOKALAN® HP 56

0.1 MPotassium/sodium phosphate7.0 14% v/v Jeffamine® ED-900 11% v/v Jeffamine® SD-2001

0.2 M Sodium malonate dibasic monohydrate 0.1M MES 5.5 20 % v/v Jeffamine® D-2000

23MBP PURIFICATION 26 NOV

PEAK FRACTIONS WERE COLLECTED AND CONCENTRATED TO 0.24 OD AND SET UP MMS3 FROM PACT MMS2

FXAC MBP PURIFICATION FPLC PEAK S75 26 NOV

PEAK FRACTIONS WERE CONCENTRATED AND CRYSTALLIZED WITH SEED STOCK FROM CS2 39 (mms2)

ANTIGEN5 MBP PURIFICATION 28 NOV

B7 TO C5 from run 1

SDS gel of FXAC MBP crystals

1                           2                    3

       1                                    2

FXAC pet28 2litre purification

fraction 4 to fraction 20 alternate even numbered fractions