GEL RUN OF CRYSTALS

 

CRYSTALS IN THE DROP 49% mpd , 600mM NH4Cl

CRYSTALS MAGNIFIED 

 

AFTER SPIN 1

 

AFTER SPIN 2

 

 

AFTER SPIN 3 

 

BEFORE ADDING DYE

 

CRYSTALS FROM OLDER PLATES SET UP ON JAN 21

BIG CRYSTALS IN ADDITIVE SCREEN OF CS2 43 AND WIZARD 3 46

DIFFRACTION SIZE CRYSTALS AT 45% MPD AND 500,600 NH4CL

SDS GEL OF CRYSTALS

 

GEL OF CRYSTALS

 

3 SUPERNATANTS 

FXAC 3L PURIFICATION AND GEL FILTRATION ON S75

      FRACTIONS A2 TO A10

CHANGED BUFFER TO 50mM TRIS PH 7.4

WIZARD 3- 46 500 NH4CL CRYSTALS GROWN BIGGER