CS2 43 ADDITIVE SCREEN AND OPTIMIZATION OF VARIOUS MPD AND HD CONDITIONS

FXAC AND 23MBP 3L PURIFICATION 31JAN

           FXAC BAW ELN1 ELN2 ELN3 BAE                          23MBP BAW ELN1 ELN2 ELN3 BAE

FXACSDS GEL OF S200 FPLC RUN OF ELUTION 1 AND 2 PEAK 1 ABOVE 7 TO 21 ALTERNATE

CRYSTALS FROM CS2 35 AND WIZ 3 46 OPTI

CRYSTALS FROM CS2 - 39 . 43 AND JCSG 11

FXAC CRYSTALLIZTION CONDITION OPTI WIZ3 46 AND CS2 35

PURIFICATION AND FPLC OF FXAC MBP 4L PELLET

FPLC OF ELUTION1 ON S75 10ML INJECTION

FPLC FRACTIONS RUN ON SDS GEL FRACTIONS 4 TO 12

SAME IMAGE BRIGHTENED

SAME GEL ON 31JAN FULLY DESTAINED IN WATER FOR FEW DAYS

ELUTIONS 2  (10ML INJECTION) S75

ELUTION 3 5ML INJECTION S75