MASS SPEC RESULT OF FXAC BAND

GEL RUN OF CRYSTALS

 

CRYSTALS IN THE DROP 49% mpd , 600mM NH4Cl

CRYSTALS MAGNIFIED 

 

AFTER SPIN 1

 

AFTER SPIN 2

 

 

AFTER SPIN 3 

 

BEFORE ADDING DYE

 

CRYSTALS FROM OLDER PLATES SET UP ON JAN 21

BIG CRYSTALS IN ADDITIVE SCREEN OF CS2 43 AND WIZARD 3 46

DIFFRACTION SIZE CRYSTALS AT 45% MPD AND 500,600 NH4CL

SDS GEL OF CRYSTALS

 

GEL OF CRYSTALS

 

3 SUPERNATANTS 

FXAC 3L PURIFICATION AND GEL FILTRATION ON S75

      FRACTIONS A2 TO A10

CHANGED BUFFER TO 50mM TRIS PH 7.4

WIZARD 3- 46 500 NH4CL CRYSTALS GROWN BIGGER

CS2-39 ADDITIVE SCREEN

FXAC PROTEASE CLEAVAGE WITH PROTIACE KITS FROM HAMPTON

NATIVE GEL-FXAC + THROMBIN BINDING ASSAY

FXAC 2 L PURIFICATION AND FPLC 9 FEB

 FPLC ELUTION 1 ON S200

FPLC ELUTION 2 ON S200

PURIFICATION SDS GEL LYS, BAW, ELN1 , ELN2 

TOP FPLC OF ELUTION 1

WIZARD 3 OPTIMIZATION CRYSTAL AT 500 NH4CL

GEL OF CRYSTAL

DIFFRACTION TESTING 7 FEB

CS2 43 ADDITIVE 5 DIAMOND SHAPED CRYSTAL

PROTEIN OR SALT TEST WITH GLUTARALDEHYDE

ADDITIVE SCREEN WITH BIG CRYSTALS